FBXO5 Recombinant monoclonal antibody

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• FBXO5 Recombinant monoclonal antibody (86814-1-RR)

• 

  WB analysis using 86814-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86814-1-RR (FBXO5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FBXO5 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86814-1-RR

Clone No.251850F4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Early mitotic inhibitor 1, EMI1, F box only protein 5, F box protein 5, F-box only protein 5

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86814-1-RR targets FBXO5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1296

  Product name: Recombinant human FBXO5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-447 aa of BC018905

  Sequence: MSRRPCSCALRPPRCSCSASPSAVTAAGRPRPSDSCKEESSTLSVKMKCDFNCNHVHSGLKLVKPDDIGRLVSYTPAYLEGSCKDCIKDYERLSCIGSPIVSPRIVELETESKRLHNKENQHVQQTLNSTNEIEALETSRLYEDSGYSSFSLQSGLSEHEEGSLLEENFGDSLQSCLLQIQSPDQYPNKNLLPVLHFEKVVCSTLKKNAKRNPKVDREMLKEIIARGNFRLQNIIGRKMGLECVDILSELFRRGLRHVLATILAQLSDMDLINVSKVSTTWKKILEDDKGAFQLYSKAIQRVTENNNKFSPHASTREYVMFRTPLASVQKSAAQTSLKKDAQTKLSNQGDQKGSTYSRHNEFSEVAKTLKKNESLKACIRCNSPAKYDCYLQRATCKREGCGFDYCTKCLCNYHTTKDCSDGKLLKASCKIGPLPGTKKSKKNLRRL

• Full Name: F-box protein 5
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC018905
• Gene Symbol: FBXO5
• Gene ID (NCBI): 26271
• RRID: AB_3745138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UKT4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXO5, also named as EMI1 and FBX5, is a key regulator of the normal cell cycle. It is a a zinc finger protein expressed by a variety of adult tissues and especially in proliferating Ki-67-positive cells. It binds to the APC activators CDC20 and FZR1/CDH1 to prevent APC activation. FBXO5 can also bind directly to the APC to inhibit substrate-binding.