FBXO7 Polyclonal antibody

FBXO7 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 10696-1-AP

Synonyms

F box only protein 7, F box protein 7, FBX, FBX07, FBX7, FBXO7, PARK15, PKPS



Tested Applications

Positive WB detected inHeLa cells, BxPC-3 cells, human brain tissue, human placenta tissue, K-562 cells, MCF-7 cells, mouse testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

10696-1-AP targets FBXO7 in WB, IP, CoIP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO7 fusion protein Ag1035
Full Name F-box protein 7
Calculated molecular weight 58.5 kDa
Observed molecular weight 58.5 kDa
GenBank accession numberBC008361
Gene symbol FBXO7
Gene ID (NCBI) 25793
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXO7, also named as FBX7, is a substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. FBXO7 recognizes BIRC2 and DLGAP5. It function in phosphorylation-dependent ubiquitination.

Protocols

Product Specific Protocols
WB protocol for FBXO7 antibody 10696-1-APDownload protocol
IP protocol for FBXO7 antibody 10696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

JCI Insight

Chemical inhibition of FBXO7 reduces inflammation and confers neuroprotection by stabilizing the mitochondrial kinase PINK1.

Authors - Yuan Liu
humanWB,CoIP

Hepatology

Novel mechanism by which histone deacetylase inhibitors facilitate topoisomerase IIα degradation in hepatocellular carcinoma cells.

Authors - Chen Mei-Chuan MC