FBXO9 Polyclonal antibody

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• FBXO9 Polyclonal antibody (11161-1-AP)

FBXO9 Polyclonal Antibody for WB, ELISA

Cat No. 11161-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Cross-immune reaction antigen 1, F box only protein 9, F box protein 9, F-box only protein 9, FBX9

• 

  WB analysis of human liver using 11161-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11161-1-AP (FBXO9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11161-1-AP targets FBXO9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1643

  Product name: Recombinant human FBXO9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 96-403 aa of BC000650

  Sequence: DGVGNSYIEDNDDDSKMADLLSYFQQQLTFQESVLKLCQPELESSQIHISVLPMEVLMYIFRWVVSSDLDLRSLEQLSLVCRGFYICARDPEIWRLACLKVWGRSCIKLVPYTSWREMFLERPRVRFDGVYISKTTYIRQGEQSLDGFYRAWHQVEYYRYIRFFPDGHVMMLTTPEEPQSIVPRLRTRNTRTDAILLGHYRLSQDTDNQTKVFAVITKKKEEKPLDYKYRYFRRVPVQEADQSFHVGLQLCSSGHQRFNKLIWIHHSCHITYKSTGETAVSAFEIDKMYTPLFFARVRSYTAFSERPL

• Full Name: F-box protein 9
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC000650
• Gene Symbol: FBXO9
• Gene ID (NCBI): 26268
• RRID: AB_2231577
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UK97
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. The F-box proteins are divided into 3 classes: FBWs containing WD-40 domains, FBLs containing leucine-rich repeats, and FBXs containing either different protein-protein interaction modules or no recognizable motifs. FBOX9 has three isoforms produced by alternative splicing with predicted MW of 38-42, 47 and 50-60 kDa.

Publications

What published studies show

The FBXO9 Polyclonal antibody (Cat# 11161-1-AP) has 1 citation. It appears in American Journal of Cancer Res (2022), where it was used for Western blot to study how the E3 ubiquitin ligase RBCK1 promotes invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex. The associated research field is cancer biology, specifically hepatocellular carcinoma and ubiquitin-mediated protein degradation pathways.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Am J Cancer Res; The E3 ubiquitin ligase RBCK1 promotes the invasion and metastasis of hepatocellular carcinoma by destroying the PPARγ/PGC1α complex.; Authors - Zheng Xu