Tested Applications
| Positive WB detected in | HEK-293 cells, mouse kidney tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11499-1-AP targets FBXW2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2045 Product name: Recombinant human FBXW2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC018738 Sequence: MERKDFETWLDNISVTFLSLTDLQKNETLDHLISLSGAVQLRHLSNNLETLLKRDFLKLLPLELSFYLLKWLDPQTLLTCCLVSKQWNKVISACTEVWQTACKNLGWQIDDSVQDALHWKKVYLKAILRMKQLEDHEAFETSSLIGHSARVYALYYKDGLLCTGSDDLSAKLWDVSTGQCVYGIQTHTCAAVKFDEQKLVTGSFDNTVACWEWSSGARTQHFRGHTGAVFSVDYNDELDILVSGSADFTVKVWALSAGTCLNTLTGHTEWVTKVVLQKCKVKSLLHSPGDYILLSADKYEIKIWPIGREINCKC Predict reactive species |
| Full Name | F-box and WD repeat domain containing 2 |
| Calculated Molecular Weight | 44 kDa, 52 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC018738 |
| Gene Symbol | FBXW2 |
| Gene ID (NCBI) | 26190 |
| RRID | AB_2102740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UKT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The human FBW2 polypeptide contains an N-terminal F-box motif and a C-terminal domain of five WD repeats. A WD repeat is a motif typically consisting of 44-60 amino acids with a signature tryptophan and aspartic acid dipeptide at its C-terminus. The WD repeats in FBW2 recognize GCM1 in a way that is facilitated by GSK3b. Two isoforms were produced by alternative splicing with predicted MW of 52 and 43 kDa according to UniProt, and Catalog#11499-1-AP can recognise both.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FBXW2 antibody 11499-1-AP | Download protocol |
| WB protocol for FBXW2 antibody 11499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-SKP2 antibody (Cat# 11499-1-AP) has 8 citations published in journals including Nature Communications, Cell Death & Disease, Cancer Letters, Cell Molecular Gastroenterology and Hepatology, Cellular and Molecular Life Sciences, American Journal of Respiratory Cell and Molecular Biology, and Journal of Biological Chemistry. Research fields span lung cancer, breast cancer, hepatocellular carcinoma, prostate cancer, cell cycle regulation, ubiquitin-proteasome pathway, and acute lung injury.
| Species | Application | Title |
|---|---|---|
Nat Commun The β-TrCP-FBXW2-SKP2 axis regulates lung cancer cell growth with FBXW2 acting as a tumour suppressor.
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Nat Commun FBXW2 suppresses migration and invasion of lung cancer cells via promoting β-catenin ubiquitylation and degradation.
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Cell Death Dis FBXW2 suppresses breast tumorigenesis by targeting AKT-Moesin-SKP2 axis
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Cancer Lett FBXO43 promotes cell cycle progression in cancer cells through stabilizing SKP2 | ||
Cell Mol Gastroenterol Hepatol TAK1 Is a Novel Target in Hepatocellular Carcinoma and Contributes to Sorafenib Resistance.
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Cell Mol Life Sci FBXW2 inhibits prostate cancer proliferation and metastasis via promoting EGFR ubiquitylation and degradation.
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