FBXW7 Polyclonal antibody

• Featured Product
• KD/KO Validated

FBXW7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (3)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

36

Cat no : 28424-1-AP

Synonyms

AGO, Archipelago homolog, CDC4, DKFZp686F23254, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, FLJ16457, hAgo, hCdc4, SEL 10, SEL10

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Validation Data Gallery

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  WB analysis using 28424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28424-1-AP (FBXW7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28424-1-AP (FBXW7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, MCF-7 cells, mouse brain tissue
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 33 publications below
• IHC: See 8 publications below
• IF: See 4 publications below
• IP: See 7 publications below

Product Information

28424-1-AP targets FBXW7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXW7 fusion protein Ag28471
• Full Name: F-box and WD repeat domain containing 7
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 100-110 kDa, 66-75 kDa
• GenBank Accession Number: NM_001349798
• Gene Symbol: FBXW7
• Gene ID (NCBI): 55294
• RRID: AB_2881138
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 31346036/PMID: 22864569). K48 autoubiquitination is consistent with maintenance of ubiquitinated FBW7 (Ub-FBW7, 200 kDa)(PMID: 32353058).

Publications

Species	Application	Title
human	WB	Mol Cancer; Loss of FBXW7-mediated degradation of BRAF elicits resistance to BET inhibitors in adult T cell leukemia cells.; Authors - Chien-Hung Yeh
human	WB,IP	J Exp Med; Somatic gain-of-function mutations in BUD13 promote oncogenesis by disrupting Fbw7 function; Authors - Jianfeng Chen
mouse	WB	Theranostics; Histone demethylase JMJD3 protects against renal fibrosis by suppressing TGFβ and Notch signaling and preserving PTEN expression.; Authors - Chao Yu
mouse	WB	EMBO Rep; The 18S rRNA m6 A methyltransferase METTL5 promotes mouse embryonic stem cell differentiation.; Authors - Ming Xing
human	WB,IP,IF	Free Radic Biol Med; NOX1 triggers ferroptosis and ferritinophagy, contributes to Parkinson's disease; Authors - Huiqing Wang
human,mouse	WB,IHC	Int Immunopharmacol; MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7; Authors - Shuhong Wu