• Featured Product
  • KD/KO Validated

FBXW7 Polyclonal antibody

FBXW7 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 55290-1-AP


AGO, Archipelago homolog, CDC4, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, hAgo, hCdc4, SEL 10, SEL10

Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman skin cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55290-1-AP targets FBXW7 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name F-box and WD repeat domain containing 7
Calculated Molecular Weight 80 kDa
Observed Molecular Weight100-110 kDa, 66-75 kDa
GenBank Accession NumberNM_018315
Gene Symbol FBXW7
Gene ID (NCBI) 55294
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.


Product Specific Protocols
WB protocol for FBXW7 antibody 55290-1-APDownload protocol
IHC protocol for FBXW7 antibody 55290-1-APDownload protocol
IF protocol for FBXW7 antibody 55290-1-APDownload protocol
IP protocol for FBXW7 antibody 55290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Cyclin E-induced replicative stress drives p53-dependent whole-genome duplication

Authors - Jingkun Zeng
  • KD Validated


Polycomb repressive complex 2 binds and stabilizes NANOG to suppress differentiation-related genes to promote self-renewal

Authors - Da-Wei Yeh


PMID: 28404959

Authors - Min Zheng


Rictor regulates spermatogenesis by controlling Sertoli cell cytoskeletal organization and cell polarity in the mouse testis.

Authors - Heling Dong

Int J Biochem Cell Biol

The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc.

Authors - Zhijun Xu

Biosci Rep

PMID: 28507201

Authors - Haiwei Zhang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Iram (Verified Customer) (09-17-2020)

FBX W7 is an excellent antibody for WB and IHC. Giving a very clear bands and sharp staining in tissue sections.

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer