FBXW7 Polyclonal antibody

FBXW7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 55290-1-AP

Synonyms

AGO, Archipelago homolog, CDC4, F box protein FBX30, FBW6, FBW7, FBX30, FBXO30, FBXW6, FBXW7, hAgo, hCdc4, SEL 10, SEL10



Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue
Positive IHC detected inhuman skin cancer tissue, human colon cancer tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 6 publications below

Product Information

55290-1-AP targets FBXW7 in WB, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name F-box and WD repeat domain containing 7
Calculated molecular weight 80 kDa
Observed molecular weight 100-110 kDa, 60-65 kDa
GenBank accession numberNM_018315
Gene symbol FBXW7
Gene ID (NCBI) 55294
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.

Protocols

Product Specific Protocols
WB protocol for FBXW7 antibody 55290-1-APDownload protocol
IHC protocol for FBXW7 antibody 55290-1-APDownload protocol
IF protocol for FBXW7 antibody 55290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MedComm (Beijing)

Demethylzeylasteral inhibits proliferation, migration, and invasion through FBXW7/c-Myc axis in gastric cancer.

Authors - Yongsen Li
ratWB

Mol Med Rep

E3 ligase Fbw7 participates in oxidative stress‑induced myocardial cell injury via interacting with Mcl‑1.

Authors - Xia Li
humanWB

Biosci Rep

PMID: 28507201

Authors - Haiwei Zhang
humanWB

Int J Biochem Cell Biol

The deubiquitinase USP38 promotes cell proliferation through stabilizing c-Myc.

Authors - Zhijun Xu
mouseWB

Endocrinology

Rictor regulates spermatogenesis by controlling Sertoli cell cytoskeletal organization and cell polarity in the mouse testis.

Authors - Heling Dong
humanWB

Oncotarget

PMID: 28404959

Authors - Min Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Iram (Verified Customer) (09-17-2020)

FBX W7 is an excellent antibody for WB and IHC. Giving a very clear bands and sharp staining in tissue sections.

  • Applications: Western Blot,Immunohistochemistry
  • Primary Antibody Dilution: 1:1000 for western and 1:100 for IHC
  • Cell Tissue Type: Human colon cancer