Validation Data Gallery
|Positive WB detected in||HepG2 cells, HEK-293 cells, MCF-7 cells, human brain tissue|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human skin cancer tissue, human colon cancer tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55290-1-AP targets FBXW7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||F-box and WD repeat domain containing 7|
|Calculated molecular weight||80 kDa|
|Observed molecular weight||100-110 kDa, 66-75 kDa|
|GenBank accession number||NM_018315|
|Gene ID (NCBI)||55294|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
FBXW7, also named as FBW7, FBX30, SEL10 and hAgo, is a substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. It probably recognizes and binds to phosphorylated target proteins. FBXW7 is involved in the degradation of cyclin-E, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. FBXW7 is a general tumor suppressor in human cancer (PMID: 17909001). FBXW7 has 3 isoforms (α/β/γ) with the calculated molecular mass of 80 kDa, 70 kDa and 66 kDa, and apparent molecular mass of 100-110 kDa and 66-75 kDa (PMID: 18559665). This antibody is specific to FBXW7 and it can recognize all the isoforms of FBXW7.
|Product Specific Protocols|
|WB protocol for FBXW7 antibody 55290-1-AP||Download protocol|
|IHC protocol for FBXW7 antibody 55290-1-AP||Download protocol|
|IF protocol for FBXW7 antibody 55290-1-AP||Download protocol|
|IP protocol for FBXW7 antibody 55290-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Iram (Verified Customer) (09-17-2020)
FBX W7 is an excellent antibody for WB and IHC. Giving a very clear bands and sharp staining in tissue sections.