×
Use Able AI chat for product recommendations

FBXW7 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.391-25 ng/mL

Cat no : MP03838-1

Synonyms

CDC4, F-box and WD-40 domain-containing protein 7, F-box protein FBX30, F-box/WD repeat-containing protein 7, FBW7

Validation Data Gallery

  • Sandwich ELISA standard curve of MP03838-1, Human FBXW7 Recombinant Matched Antibody Pair - PBS only. 88954-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag28460. 88954-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

    Sandwich ELISA standard curve of MP03838-1

    Sandwich ELISA standard curve of MP03838-1, Human FBXW7 Recombinant Matched Antibody Pair - PBS only. 88954-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag28460. 88954-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

  • The mean FBXW7 concentration was determined to be 80.53 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load, 104.83 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 82.72 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 50.78 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load.

    Sandwich ELISA sample test of MP03838-1

    The mean FBXW7 concentration was determined to be 80.53 ng/mL in HepG2 cell extract based on a 1.20 mg/mL extract load, 104.83 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load, 82.72 ng/mL in MCF-7 cell extract based on a 1.20 mg/mL extract load and 50.78 ng/mL in A431 cell extract based on a 1.20 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP03838-1 targets FBXW7 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.391-25 ng/mL (Sandwich ELISA);

Capture antibody: FBXW7 Recombinant monoclonal antibody, PBS Only (Capture) 88954-3-PBS (261793C9). 100 μg. Concentration 1 mg/ml.

Detection antibody: FBXW7 Recombinant monoclonal antibody, PBS Only (Detector) 88954-2-PBS (261793A2). 100 μg. Concentration 1 mg/ml.

Alternative FBXW7 matched antibody pairs: MP51687-1

Matched pair protein standard: Ag28460

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 88954-3-PBS
Tested Reactivity human
Immunogen FBXW7 fusion protein Ag28460
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name F-box and WD repeat domain containing 7
Synonyms
GenBank accession numberNM_001349798
Gene symbol FBXW7
UniprotQ969H0
Gene ID (NCBI) 55294
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 88954-2-PBS
Tested Reactivity human
Immunogen FBXW7 fusion protein Ag28460
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name F-box and WD repeat domain containing 7
Synonyms
GenBank accession numberNM_001349798
Gene symbol FBXW7
UniprotQ969H0
Gene ID (NCBI) 55294
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
||
New chat

Able™

正在加载，请稍候...