FBXW8 Polyclonal antibody

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• FBXW8 Polyclonal antibody (33578-1-AP)

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  WB analysis using 33578-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33578-1-AP (FBXW8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FBXW8 Polyclonal Antibody for WB, ELISA

Cat No. 33578-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

F-box and WD-40 domain-containing protein 8, F-box only protein 29, F-box/WD repeat-containing protein 8, FBW6, FBW8

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Tested Applications

• Positive WB detected in: MCF-7 cells, MDA-MB-231 cell, U-87 MG cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33578-1-AP targets FBXW8 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36986

  Product name: Recombinant human FBXW8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-303 aa of BC037296

  Sequence: MDDYSLDEFRRRWQEELAQAQAPKKRRRPEAAERRARRPEVGSGRSEQASGDPALAQGLLEGAGRPPAARATRAEGQDVASRSRSPLAREGAGGGEQLVDQLIRDLNEMNDVPFFDIQLPYELAINIFQYLDRKELGRCAQVSKTWKVIAEDEVLWYRLCQQEGHLPDSSISDYSCWKLIFQECRAKEHMLQTNWKNRKGAVSELEHVPDTVLCDVHSHDGVVIAGYTSGDVRVWDTRTWDYVAPFLESEDEEDEPGMQPNVSFVRINSSLAVAAYEDGFLNIWDLRTGKYPVHRFEHDARIQ

• Full Name: F-box and WD repeat domain containing 8
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa, 60 kDa
• GenBank Accession Number: BC037296
• Gene Symbol: FBXW8
• Gene ID (NCBI): 26259
• RRID: AB_3743018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N3Y1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fbxw8 (also known as Fbw6 or Fbx29) is an F-box protein that is assembled with Cul7 in an SCF-like E3 complex. FBXW8 directs the proteasomal degradation of β-TrCP1 in MAPK pathway-dependent manner, and the attenuation of β-TrCP1 by FBXW8 is important for Cdc25A-mediated cell cycle transition from G1 phase to S phase as well as DNA damage-free progression of S phase (PMID: 33742524).