FCGR2A / CD32a Polyclonal antibody

FCGR2A / CD32a Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (2)





Cat no : 15625-1-AP


CD32, CD32A, CDw32, Fc gamma RII a, Fc gamma RIIa, FCG2, FcGR, FCGR2, FCGR2A, FCGR2A1, FcRII a, IGFR2, IgG Fc receptor II a

Tested Applications

Positive WB detected inK-562 cells, U-937 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inK-562 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15625-1-AP targets FCGR2A / CD32a in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCGR2A / CD32a fusion protein Ag8072
Full Name Fc fragment of IgG, low affinity IIa, receptor (CD32)
Calculated Molecular Weight 35 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC020823
Gene Symbol FCGR2A
Gene ID (NCBI) 2212
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IgG Fc receptors (FcγRs) play important roles in immune responses. Low affinity IgG Fc receptor Fc gamma RIIa (FCGR2A, CD32a) is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes.


Product Specific Protocols
WB protocol for FCGR2A / CD32a antibody 15625-1-APDownload protocol
IHC protocol for FCGR2A / CD32a antibody 15625-1-APDownload protocol
FC protocol for FCGR2A / CD32a antibody 15625-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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