FCGR3B/CD16b Polyclonal antibody

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  WB analysis using 30201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30201-1-AP (FCGR3B/CD16b antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung using 30201-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30201-1-AP (FCGR3B/CD16b antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 30201-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 30201-1-AP (FCGR3B/CD16b antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 30201-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FCGR3B/CD16b antibody (30201-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

FCGR3B/CD16b Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30201-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CD16, CD16b, FCGR3B, Fc gamma RIII, Fc gamma RIII beta

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, U-937 cells
• Positive IHC detected in: human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30201-1-AP targets FCGR3B/CD16b in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: NM_000570
• Gene Symbol: FCGR3B
• Gene ID (NCBI): 2215
• RRID: AB_3086262
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75015
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3B/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B/CD16b.