|Positive WB detected in||THP-1 cells, human placenta tissue, human liver tissue|
|Positive IHC detected in||human spleen tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:500-1:2000|
|Sample-dependent, check data in validation data gallery|
16190-1-AP targets FcRn-Specific in WB, IHC, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||canine, human, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||Fc fragment of IgG, receptor, transporter, alpha|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||35-40 kDa|
|GenBank accession number||NM_004107|
|Gene ID (NCBI)||2217|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).
Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.
Clin Dev Immunol
Human antibodies can cross guinea pig placenta and bind its neonatal Fc Receptor: implications for studying immune prophylaxis and therapy during pregnancy.
Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.