FcRn-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

FcRn-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 16190-1-AP

Synonyms

alpha chain, FCGRT, FCRN, FcRn-Specific, Neonatal Fc receptor

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Validation Data Gallery

• 

  WB analysis using 16190-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16190-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16190-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16190-1-AP (FcRn-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16190-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16190-1-AP (FcRn-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: THP-1 cells, human placenta tissue, human liver tissue
• Positive IHC detected in: human spleen tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 2 publications below

Product Information

16190-1-AP targets FcRn-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: Fc fragment of IgG, receptor, transporter, alpha
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: NM_004107
• Gene Symbol: FcRn
• Gene ID (NCBI): 2217
• RRID: AB_2231751
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).

Publications

Species	Application	Title
human	WB	Cell; Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.; Authors - Xin Zhao ; KO Validated
human	IF	Front Immunol; Impact of mAb-FcRn affinity on IgG transcytosis across human well-differentiated airway epithelium; Authors - Kohei Togami
pig	WB	Clin Dev Immunol; Human antibodies can cross guinea pig placenta and bind its neonatal Fc Receptor: implications for studying immune prophylaxis and therapy during pregnancy.; Authors - Struble Evi Budo EB
canine	WB,IF	Placenta; Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.; Authors - Yanqun Xu
human	WB	Int J Mol Sci; Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes; Authors - Zhonghao Li
		J Agric Food Chem; Prevention of Hypercholesterolemia with "Liposomes in Microspheres" Composite Carriers: A Promising Approach for Intestinal-Targeted Oral Delivery of Astaxanthin; Authors - Aiyang Liu

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