• Featured Product
  • KD/KO Validated

FcRn-Specific Polyclonal antibody

FcRn-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16190-1-AP

Synonyms

alpha chain, FCGRT, FCRN, Neonatal Fc receptor



Tested Applications

Positive WB detected inTHP-1 cells, human placenta tissue, human liver tissue
Positive IHC detected inhuman spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

16190-1-AP targets FcRn-Specific in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity canine, human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name Fc fragment of IgG, receptor, transporter, alpha
Calculated molecular weight 40 kDa
Observed molecular weight 35-40 kDa
GenBank accession numberNM_004107
Gene symbol FCGRT
Gene ID (NCBI) 2217
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).

Protocols

Product Specific Protocols
WB protocol for FcRn-Specific antibody 16190-1-APDownload protocol
IHC protocol for FcRn-Specific antibody 16190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB,IF

Placenta

Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.

Authors - Yanqun Xu
pigWB

Clin Dev Immunol

Human antibodies can cross guinea pig placenta and bind its neonatal Fc Receptor: implications for studying immune prophylaxis and therapy during pregnancy.

Authors - Struble Evi Budo EB
humanWB

Cell

Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.

Authors - Xin Zhao
  • KO Validated