|Positive WB detected in
|THP-1 cells, human placenta tissue, human liver tissue
|Positive IHC detected in
|human spleen tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:500-1:2000
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
16190-1-AP targets FcRn-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|human, canine, pig
|Host / Isotype
|Rabbit / IgG
|Fc fragment of IgG, receptor, transporter, alpha
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
The neonatal Fc receptor for IgG (FcRn) is a membrane-associated receptor which acts as a key determinant of IgG homeostasis. FcRn is similar in structure to major histocompatibility class I molecules. It is a heterodimer composed of a alpha chain associated with β2-microglobulin. FcRn transfers IgG from the mother to the fetus across the placenta and the proximal small intestine. In addition, throughout life, FcRn protects IgG from degradation and thus regulates the serum half-life of IgG (PMID: 17703228).
Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B.
Clin Dev Immunol
Human antibodies can cross guinea pig placenta and bind its neonatal Fc Receptor: implications for studying immune prophylaxis and therapy during pregnancy.
Gestation age dependent transfer of human immunoglobulins across placenta in timed-pregnant guinea pigs.
Int J Mol Sci
Recombinant Humanized IgG1 Antibody Promotes Reverse Cholesterol Transport through FcRn-ERK1/2-PPARα Pathway in Hepatocytes