FCN1 Polyclonal antibody

FCN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11775-1-AP

Synonyms

Ficolin1, Ficolin alpha, Ficolin A, Ficolin 1, FCN 1

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Validation Data Gallery

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  WB analysis using 11775-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11775-1-AP (FCN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11775-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11775-1-AP (FCN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11775-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11775-1-AP (FCN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: human plasma, K-562 cells, human liver tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11775-1-AP targets FCN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FCN1 fusion protein Ag2444
• Full Name: ficolin (collagen/fibrinogen domain containing) 1
• Calculated Molecular Weight: 326 aa, 35 kDa
• Observed Molecular Weight: 33-37 kDa
• GenBank Accession Number: BC020635
• Gene Symbol: FCN1
• Gene ID (NCBI): 2219
• RRID: AB_2104288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Immunol; The complex formation of MASP-3 with pattern recognition molecules of the lectin complement pathway retains MASP-3 in the circulation; Authors - Kohei Kusakari
human	IHC	Front Immunol; A novel necroptosis-related gene index for predicting prognosis and a cold tumor immune microenvironment in stomach adenocarcinoma; Authors - Muhammad Khan