FDX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FDX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

61

Cat no : 12592-1-AP

Synonyms

Ferredoxin-1, FDX, ADX

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Validation Data Gallery

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  WB analysis using 12592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12592-1-AP

  WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HEK-293 cells.

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  WB analysis of HepG2 using 12592-1-AP

  WB result of FDX1 antibody (12592-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FDX1 transfected HepG2 cells.

• 

  WB analysis using 12592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12592-1-AP (FDX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12592-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12592-1-AP (FDX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDX1 antibody (12592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HepG2 cells, PC-3 cells, mouse testis tissue
• Positive IHC detected in: human kidney tissue, human liver tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 50 publications below
• IHC: See 21 publications below
• IF: See 7 publications below

Product Information

12592-1-AP targets FDX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FDX1 fusion protein Ag3301
• Full Name: ferredoxin 1
• Calculated Molecular Weight: 184 aa, 19 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC017063
• Gene Symbol: FDX1
• Gene ID (NCBI): 2230
• RRID: AB_11182486
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FDX1(Ferredoxin-1) is also named as ADX, FDX, YAH1, LOH11CR1D and belongs to the adrenodoxin/putidaredoxin family. It is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450 and it can only reduce mitochondrial CYP enzymes that are essential in adrenal steroidogenesis, bile acid formation, and vitamin D synthesis. The full length has a transit peptide of 60 amino acids.

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	Nat Chem Biol; Mitochondrial metabolism promotes adaptation to proteotoxic stress.; Authors - Peter Tsvetkov ; KO Validated
mouse	WB,IF	Adv Healthc Mater; Copper-Based Composites Nanoparticles Improve Triple-Negative Breast Cancer Treatment with Induction of Apoptosis-Cuproptosis and Immune Activation; Authors - Ning Wang
human	WB	J Biol Chem; Lipoylation is dependent on the ferredoxin FDX1 and dispensable under hypoxia in human cells; Authors - Pallavi R Joshi
human	WB	Front Oncol; ITGB1-mediated molecular landscape and cuproptosis phenotype induced the worse prognosis in diffuse gastric cancer; Authors - Xingyu Zhu