FEM1B Polyclonal antibody

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• FEM1B Polyclonal antibody (19544-1-AP)

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• KD/KO Validated

FEM1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19544-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FEM1-beta, FEM1 beta, fem 1 homolog b (C. elegans), F1A-alpha, F1AA

• 

  WB analysis of PC-3 using 19544-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse pancreas using 19544-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse testis using 19544-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of RAW264.7 using 19544-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 19544-1-AP (FEM1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 19544-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 19544-1-AP (FEM1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of PC-3 using 19544-1-AP

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using FEM1B antibody (19544-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse pancreas tissue, mouse testis tissue
• Positive IHC detected in: human pancreas cancer tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19544-1-AP targets FEM1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: fem-1 homolog b (C. elegans)
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: NM_015322
• Gene Symbol: FEM1B
• Gene ID (NCBI): 10116
• RRID: AB_10644294
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UK73
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEM1B, also named F1AA and KIAA0396, belongs to the fem-1 family. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. It is involved in glucose homeostasis in pancreatic islets.

Publications

What published studies show

The FEM1B Polyclonal antibody (Cat# 19544-1-AP) has 7 citations published in Cell (2), Molecular Cell (2), Journal of the American Chemical Society, Nature Chemical Biology, and Journal of Medicinal Chemistry. Research fields span reductive stress detection, targeted protein degradation (PROTACs), ferroptosis regulation in lung cancer, mitochondrial protein import, and E3 ligase-mediated mitochondrial dynamics. Applications include Western blot, IHC, and IF in human and mouse models.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Cell; Structural basis and regulation of the reductive stress response; Authors - Andrew G Manford
mouse	WB	Cell; A Cellular Mechanism to Detect and Alleviate Reductive Stress.; Authors - Andrew G Manford ; KD Validated
human	WB	J Am Chem Soc; Discovery of a Covalent FEM1B Recruiter for Targeted Protein Degradation Applications; Authors - Nathaniel J. Henning
human	WB,IF	Mol Cell; Reactive oxygen species control protein degradation at the mitochondrial import gate; Authors - Rachael McMinimy
human,mouse	WB,IHC	Mol Cell; CRL2(FEM1B) uses heme to recruit BACH1 for degradation and regulate ferroptosis in lung cancer.; Authors - Bashir Ahmed
	WB	Nat Chem Biol; TOM20-driven E3 ligase recruitment regulates mitochondrial dynamics through PLD6; Authors - Anat Raiff