FEN1 Recombinant antibody

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  WB analysis using 84916-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84916-6-RR (FEN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84916-6-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FEN1 antibody (84916-6-RR, Clone: 242330E12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84916-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84916-6-RR against Human FEN1 were performed. The affinity constant is 10.3 nM.

FEN1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84916-6-RR

Clone No.242330E12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

hFEN 1, Flap structure-specific endonuclease 1, FEN-1, FEN 1, EC:3.1.-.-

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells. HeLa cells, Y79 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84916-6-RR targets FEN1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FEN1 fusion protein Ag6552
• Full Name: flap structure-specific endonuclease 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000323
• Gene Symbol: FEN1
• Gene ID (NCBI): 2237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P39748
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).