Tested Applications
| Positive WB detected in | A549 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:14000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11453-1-AP targets Kindlin 2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2013 Product name: Recombinant human Kindlin 2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 331-680 aa of BC017327 Sequence: TSENHLNNSDKEVDEVDAALSDLEITLEGGKTSTILGDITSIPELADYIKVFKPKKLTLKGYKQYWCTFKDTSISCYKSKEESSGTPAHQMNLRGCEVTPDVNISGQKFNIKLLIPVAEGMNEIWLRCDNEKQYAHWMAACRLASKGKTMADSSYNLEVQNILSFLKMQHLNPDPQLIPEQITTDITPECLVSPRYLKKYKNKQITARILEAHQNVAQMSLIEAKMRFIQAWQSLPEFGITHFIARFQGGKKEELIGIAYNRLIRMDASTGDAIKTWRFSNMKQWNVNWEIKMVTVEFADEVRLSFICTEVDCKVVHEFIGGYIFLSTRAKDQNESLDEEMFYKLTSGWV Predict reactive species |
| Full Name | fermitin family homolog 2 (Drosophila) |
| Calculated Molecular Weight | 680 aa, 78 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC017327 |
| Gene Symbol | Kindlin 2 |
| Gene ID (NCBI) | 10979 |
| RRID | AB_2262660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96AC1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| IP protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| WB protocol for Kindlin 2 antibody 11453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Kindlin 2 Polyclonal antibody (Cat# 11453-1-AP) has 46 citations across journals including Science, Nature Communications, Cancer Research, Circulation Research, Cell Reports, Journal of Cell Biology, EMBO Reports, Journal of Biological Chemistry, and eLife. Research fields span cancer biology (pancreatic, breast, cervical, gastric, lung), fibrosis (hepatic, pulmonary, renal), integrin signaling and mechanotransduction, cell migration and adhesion, cardiac structure/function, ferroptosis, osteogenesis, and stem cell biology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Engineered extracellular vesicles for targeted reprogramming of cancer-associated fibroblasts to potentiate therapy of pancreatic cancer | ||
Science Molecular basis of astrocyte diversity and morphology across the CNS in health and disease | ||
Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis | ||
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. | ||
Circ Res Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.
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Reviews
What customers say
The single review for this antibody is highly positive, rated 5 stars. The reviewer used it for Western Blot and Immunofluorescence on HeLa and NIH3T3 cells, with dilutions of 1:1000 for WB and 1:100 for IF. They described it as an excellent antibody and highly recommended it for Western Blot applications. Overall, the feedback reflects strong satisfaction with the product's performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Yasmin (Verified Customer) (08-01-2019) | Excellent antibody, highly recommended for western blot.
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