Kindlin 2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Kindlin 2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

36

Reviews (1)

Cat no : 11453-1-AP

Synonyms

Fermitin family homolog 2, FERMT2, KIND2, Kindlin 2, mig 2, MIG2, PLEKHC1, UNC112, UNC112B

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Validation Data Gallery

• 

  WB analysis of A549 using 11453-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11453-1-AP (Kindlin 2 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11453-1-AP

  WB result of Kindlin 2 antibody (11453-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Kindlin 2 transfected A549 cells.

• 

  IP experiment of A549 using 11453-1-AP

  IP result of anti-Kindlin 2 (IP:11453-1-AP, 3ug; Detection:11453-1-AP 1:1000) with A549 cells lysate 3300ug.

• 

  IHC staining of human lung cancer using 11453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11453-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 11453-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11453-1-AP (Kindlin 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 13 publications below
• WB: See 30 publications below
• IHC: See 10 publications below
• IF: See 13 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below

Product Information

11453-1-AP targets Kindlin 2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, zebrafish, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kindlin 2 fusion protein Ag2013
• Full Name: fermitin family homolog 2 (Drosophila)
• Calculated Molecular Weight: 680 aa, 78 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC017327
• Gene Symbol: Kindlin 2
• Gene ID (NCBI): 10979
• RRID: AB_2262660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FERMT2, also named as KIND2, MIG2, PLEKHC1, MIG-2 and Kindlin-2, belongs to the kindlin family. There are three FERMT2 isoforms that participate in the connection between extracellular matrix (ECM) adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. FERMT2 recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites. Variable expression in human cancers and mesenchymal cancer invasion are now linked with FERMT2, which may plays important role in carcinormas development. Catalog#11453-1-AP is a rabbit polyclonal antibody against human FERMT2.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth.; Authors - Ling Guo
mouse, zebrafish	WB, IF	Circ Res; Kindlin-2 is an essential component of intercalated discs and is required for vertebrate cardiac structure and function.; Authors - James J Dowling ; KD Validated
zebrafish	IF	Cancer Res; Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis; Authors - Ying Liu
mouse,human	WB,IP	J Am Soc Nephrol; Kindlin-2 Association with Rho GDP-Dissociation Inhibitor α Suppresses Rac1 Activation and Podocyte Injury.; Authors - Ying Sun ; KD Validated
human	WB, IF	J Cell Sci; Differences in binding to the ILK complex determines kindlin isoform adhesion localization and integrin activation.; Authors - Clotilde Huet-Calderwood ; KD Validated
human	WB	J Cell Sci; Kindlin-2 promotes rear focal adhesion disassembly and directional persistence in cell migration; Authors - Jie Liu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yasmin (Verified Customer) (08-01-2019)

Excellent antibody, highly recommended for western blot.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 for western blot, 1:100 for IF
• Cell Tissue Type: HeLa, NIH3T3

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