FES Polyclonal antibody

FES Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28194-1-AP

Synonyms

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

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Validation Data Gallery

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  WB analysis using 28194-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28194-1-AP (FES antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 28194-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HL-60 cells, THP-1 cells, U-937 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28194-1-AP targets FES in WB, IHC,ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FES fusion protein Ag28095
• Full Name: feline sarcoma oncogene
• Calculated Molecular Weight: 822 aa, 94 kDa
• Observed Molecular Weight: 93 kDa, 79 kDa, 87 kDa, 85 kDa
• GenBank Accession Number: BC035357
• Gene Symbol: FES
• Gene ID (NCBI): 2242
• RRID: AB_2881086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025).