FFAR2 Polyclonal antibody

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  WB analysis using 19952-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 19952-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 19952-1-AP

  pig liver tissue were subjected to SDS PAGE followed by western blot with 19952-1-AP (FFAR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 19952-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19952-1-AP (FFAR2 Antibody) at dilution of 1:100 (under 40x lens).

• Featured Product
• KD/KO Validated

FFAR2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 19952-1-AP

Publications(33)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, pig and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FFA2, FFA2R, free fatty acid receptor 2, G protein coupled receptor 43, GPCR43

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Tested Applications

• Positive WB detected in: THP-1 cells, HCT 116 cells, pig liver tissue, SW 480 cells, HeLa cells
• Positive IHC detected in: human spleen tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 28 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Product Information

19952-1-AP targets FFAR2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: free fatty acid receptor 2
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 50 kDa, 37 kDa
• GenBank Accession Number: NM_005306
• Gene Symbol: FFAR2
• Gene ID (NCBI): 2867
• RRID: AB_2878628
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15552
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Food Chem X; Dendrobium officinale leaf polysaccharides ameliorated hyperglycemia and promoted gut bacterial associated SCFAs to alleviate type 2 diabetes in adult mice.; Authors - Jingyu Fang
rat	WB	Chin Med; The relationship between gut microbiota and susceptibility to type 2 diabetes mellitus in rats; Authors - Yongcheng An
human	WB	Chin Med; Shaoyao Decoction reduced T lymphocyte activation by regulating of intestinal flora and 5-hydroxytryptamine metabolism in ulcerative colitis; Authors - Jianhua Zhen
mouse	WB,IHC	Pharmaceuticals (Basel); Xiao Cheng Qi Decoction, an Ancient Chinese Herbal Mixture, Relieves Loperamide-Induced Slow-Transit Constipation in Mice: An Action Mediated by Gut Microbiota; Authors - Amanguli Tuohongerbieke
rat	WB	Phytomedicine; The beneficial effects of Polygonatum sibiricum Red. superfine powder on metabolic hypertensive rats via gut-derived LPS/TLR4 pathway inhibition; Authors - Jie Su
mouse	WB	Food Funct; EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.; Authors - Jian Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (01-26-2025)

Antibody used to study G protein coupled receptors responsible for uptake of short chain fatty acids in human iPSC-derived microglia and macrophages.

• Applications: Western Blot
• Primary Antibody Dilution: 1:750 in milk
• Cell Tissue Type: Human iPSC-derived macrophages and microglia