FFAR2 Recombinant monoclonal antibody

• 

  WB analysis using 84544-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84544-1-RR (FFAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FFAR2 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84544-1-RR

Clone No.241696B4

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Synonyms

241696B4, FFA2, FFA2R, free fatty acid receptor 2, G protein coupled receptor 43

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Tested Applications

• Positive WB detected in: THP-1 cells, HCT 116 cells, HT-29 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IP: See 1 publications below

Product Information

84544-1-RR targets FFAR2 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: free fatty acid receptor 2
• Calculated Molecular Weight: 330 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC096198
• Gene Symbol: FFAR2
• Gene ID (NCBI): 2867
• RRID: AB_3672049
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15552
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; Gut Microbiota-Derived Propionic Acid Mediates ApoA-I-Induced Amelioration of MASLD via Activation of GPR43-Ca(2+)-CAMKII-ATGL Hepatic Lipolysis.; Authors - Mengyuan Liu
mouse	WB	Transl Neurodegener; Bacteroides coprocola protects dopaminergic neurons in rotenone-induced Parkinson's disease mouse model by modulating gut microbiota dysbiosis and inhibiting the NLRP3 signaling pathway.; Authors - Zixian Liu
human	IP	J Exp Clin Cancer Res; Colon-Targeted astragalus polysaccharide nanoparticles prevent NAFLD-Driven hepatocarcinogenesis via microbiota remodeling and NF-κB Inhibition.; Authors - Dan Liu