Fibrinogen Beta Chain Polyclonal antibody

• Featured Product
• KD/KO Validated

Fibrinogen Beta Chain Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 16747-1-AP

Synonyms

FGB, fibrinogen beta chain

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Validation Data Gallery

• 

  WB analysis of mouse heart using 16747-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16747-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16747-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16747-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 16747-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 16747-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16747-1-AP (Fibrinogen beta chain antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse kidney tissue, mouse brain tissue, mouse liver tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

The immunogen of 16747-1-AP is Fibrinogen Beta Chain Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, Plasmodium falciparum
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Fibrinogen Beta Chain fusion protein Ag10169
• Full Name: fibrinogen beta chain
• Calculated Molecular Weight: 491 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC107766
• Gene Symbol: FGB
• Gene ID (NCBI): 2244
• RRID: AB_11042764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

Publications

Species	Application	Title
human,rat	IHC,IF	Blood; Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.; Authors - Krishnamoorthy Aparna A
human	WB,IHC,IF	Front Neurosci; Fibrinogen Chains Intrinsic to the Brain.; Authors - Eugene V Golanov ; KD Validated
	WB,IF	Reproduction; Formation of fibrin at sights of conceptus adhesion in the ewe.; Authors - Yuta Matsuno
	WB	Biochem Biophys Res Commun; Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period.; Authors - Yuta Matsuno
Plasmodium falciparum	WB	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
mouse,human	IF	Brain Res; Accelerated accumulation of fibrinogen peptide chains with Aβ deposition in Alzheimer's disease (AD) mice and human AD brains.; Authors - Zhihong Bian

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