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Fibrinogen Beta Chain Polyclonal antibody

Fibrinogen Beta Chain Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16747-1-AP

Synonyms

FGB, fibrinogen beta chain



Tested Applications

Positive WB detected inmouse heart tissue, mouse kidney tissue, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16747-1-AP is Fibrinogen Beta Chain Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, Plasmodium falciparum, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibrinogen Beta Chain fusion protein Ag10169
Full Name fibrinogen beta chain
Calculated molecular weight 491 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC107766
Gene symbol FGB
Gene ID (NCBI) 2244
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGB is the beta chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide B is cleaved from FGB by thrombin. Mutations in the gene of FGB lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency.

Protocols

Product Specific Protocols
WB protocol for Fibrinogen Beta Chain antibody 16747-1-APDownload protocol
IHC protocol for Fibrinogen Beta Chain antibody 16747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Int J Lab Hematol

Clinical application of a new latex photometric immunoassay reagent, LPIA-GENESIS D-dimer, and its performance in patient-derived plasma samples.

Authors - Yutaka Nagahama
Plasmodium falciparumWB

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan
humanWB,IHC,IF

Front Neurosci

Fibrinogen Chains Intrinsic to the Brain.

Authors - Eugene V Golanov
  • KD Validated
human,ratIHC,IF

Blood

Fibrinogen β-derived Bβ(15-42) peptide protects against kidney ischemia/reperfusion injury.

Authors - Krishnamoorthy Aparna A