FGD5 Polyclonal antibody

• Featured Product
• KD/KO Validated

FGD5 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 20910-1-AP

Synonyms

FGD5, ZFYVE23

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Validation Data Gallery

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  IHC staining of human lung using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20910-1-AP (FGD5 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 20910-1-AP (FGD5 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20910-1-AP (FGD5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20910-1-AP (FGD5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 20910-1-AP

  Immunofluorescent analysis of HepG2 cells, using FGD5 antibody 20910-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HeLa using 20910-1-AP

  Immunofluorescent analysis of Hela cells, using FGD5 antibody 20910-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive IHC detected in: human lung tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 20910-1-AP is FGD5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGD5 fusion protein Ag15037
• Full Name: FYVE, RhoGEF and PH domain containing 5
• Calculated Molecular Weight: 1462 aa, 160 kDa
• GenBank Accession Number: BC035364
• Gene Symbol: FGD5
• Gene ID (NCBI): 152273
• RRID: AB_10733757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGD5 (or ZFYVE23) is an endothelial specific gene, plays a crucial role in vascular pruning. Deficient expression of FGD5 markedly inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model. FGD5-deficient endothelial cells fail to migrate. Moreover, the cells are susceptible to proapoptotic stimuli associated with VEGF-stimulated activation of Akt. FGD5 promotes apoptosis-induced vaso-obliteration via induction of the hey1-p53 pathway by direct binding and activation of CDC42.

Publications

Species	Application	Title
human,mouse	WB,IF,CoIP	EMBO Rep; VE-PTP inhibition stabilizes endothelial junctions by activating FGD5.; Authors - Laura J Braun ; KD Validated
human	WB	Arterioscler Thromb Vasc Biol; Facio-genital dysplasia-5 regulates matrix adhesion and survival of human endothelial cells.; Authors - Nakhaei-Nejad Maryam M
human	WB,IP	Arterioscler Thromb Vasc Biol; FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.; Authors - Maikel A Farhan ; KD Validated
human		EMBO Rep; VE-PTP inhibition stabilizes endothelial junctions by activating FGD5.; Authors - Laura J Braun
mouse	IF	Dev Cell; FGD5 in basal cells induces CXCL14 secretion that initiates a feedback loop to promote murine mammary epithelial growth and differentiation; Authors - Tingting Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Antibody performs poorly via western blot with using Near Infrared IRDye secondary antibodies.

• Applications: Western Blot
• Primary Antibody Dilution: 1 in 1000
• Cell Tissue Type: HUVEC

FGD5 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:20910-1-AP)