FGF18 Polyclonal antibody

FGF18 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 11495-1-AP

Synonyms

FGF 18, FGF18, fibroblast growth factor 18, ZFGF5

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Validation Data Gallery

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  IHC staining of mouse kidney using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse embryo using 11495-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11495-1-AP (FGF18 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11495-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGF18 antibody (11495-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive IHC detected in: mouse kidney tissue, mouse embryo tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

11495-1-AP targets FGF18 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF18 fusion protein Ag2041
• Full Name: fibroblast growth factor 18
• Calculated Molecular Weight: 207 aa, 24 kDa
• GenBank Accession Number: BC006245
• Gene Symbol: FGF18
• Gene ID (NCBI): 8817
• RRID: AB_2877770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF18 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that this protein is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Knockout studies of the similar gene in mice implied the role of this protein in regulating proliferation and differentiation of midline cerebellar structures.

Publications

Species	Application	Title
mouse	WB, IHC	Front Pharmacol; Suppression of FGF5 and FGF18 Expression by Cholesterol-Modified siRNAs Promotes Hair Growth in Mice.; Authors - Jungang Zhao
human	IHC	J Exp Clin Cancer Res; HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway.; Authors - Kai Guo
mouse	WB,IF	Ann Transl Med; Cucurbitacin promotes hair growth in mice by inhibiting the expression of fibroblast growth factor 18; Authors - Keke Guo