Tested Applications
| Positive IHC detected in | human stomach tissue, human placenta tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18171-1-AP targets FGF5 in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rabbit, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12824 Product name: Recombinant human FGF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 16-268 aa of BC074858 Sequence: LSAWAHGEKRLAPKGQPGPAATDRNPRGSSSRQSSSSAMSSSSASSSPAASLGSQGSGLEQSSFQWSPSGRRTGSLYCRVGIGFHLQIYPDGKVNGSHEANMLSVLEIFAVSQGIVGIRGVFSNKFLAMSKKGKLHASAKFTDDCKFRERFQENSYNTYASAIHRTEKTGREWYVALNKRGKAKRGCSPRVKPQHISTHFLPRFKQSEQPELSFTVTVPEKKKPPSPIKPKIPLSAPRKNTNSVKYRLKFRFG Predict reactive species |
| Full Name | fibroblast growth factor 5 |
| Calculated Molecular Weight | 268 aa, 30 kDa |
| GenBank Accession Number | BC074858 |
| Gene Symbol | FGF5 |
| Gene ID (NCBI) | 2250 |
| RRID | AB_2878511 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P12034 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGF5 antibody 18171-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FGF5 Polyclonal antibody (Cat# 18171-1-AP) has 14 citations published in journals including Int J Biol Sci, Chin Med J, Mol Ther Nucleic Acids, Commun Biol, Front Pharmacol, Hum Gene Ther, iScience, Genes (Basel), Biochem Biophys Res Commun, Mol Genet Genomics, J Endovasc Ther, and Genet Mol Res. Research fields span CRISPR gene editing, hair follicle biology, hepatocyte apoptosis, cardiac pyroptosis in sepsis, acute lung injury, preeclampsia genetics, atherosclerosis, stem cell self-renewal, and germ cell differentiation.
| Species | Application | Title |
|---|---|---|
Chin Med J (Engl) Fibroblast growth factor 5 overexpression ameliorated lipopolysaccharide-induced apoptosis of hepatocytes through regulation of the phosphoinositide-3-kinase/protein kinase B pathway | ||
Mol Ther Nucleic Acids CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations.
| ||
Commun Biol Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells. | ||
Front Pharmacol Suppression of FGF5 and FGF18 Expression by Cholesterol-Modified siRNAs Promotes Hair Growth in Mice. | ||
Hum Gene Ther Establishment of Functional Genomics Pipeline in Mouse Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9 |
