FGF8 Polyclonal antibody

FGF8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20711-1-AP

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, Fibroblast growth factor 8, HBGF 8, KAL6



Tested Applications

Positive WB detected inmouse liver tissue, L02 cells, HeLa cells, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20711-1-AP targets FGF8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name fibroblast growth factor 8 (androgen-induced)
Calculated Molecular Weight 27 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberNM_006119
Gene Symbol FGF8
Gene ID (NCBI) 2253
RRIDAB_10733225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF8, also named as AIGF and HBGF-8, belongs to the heparin-binding growth factors family. FGF8 stimulates growth of the cells in an autocrine manner. It mediates hormonal action on the growth of cancer cells. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). The antibody is specific to FGF8.

Protocols

Product Specific Protocols
WB protocol for FGF8 antibody 20711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Carcinogenesis

The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF.

Authors - Fabao Liu
humanIF

Oncoimmunology

Immunoreactivity against fibroblast growth factor 8 in alveolar rhabdomyosarcoma patients and its involvement in tumor aggressiveness.

Authors - Elena Poli
humanIHC

Oncol Lett

Systematic analysis of expression profiles and prognostic significance of the FGF gene family in pancreatic adenocarcinoma

Authors - Yu-Xin Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Paolo (Verified Customer) (12-12-2019)

It works fine in wb, in IF differences in FGF8 intracellular localization were observed according to the fixative solution used

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1/500