FGFBP1 Recombinant monoclonal antibody

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• FGFBP1 Recombinant monoclonal antibody (85879-2-RR)

• 

  WB analysis using 85879-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85879-2-RR (FGFBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 85879-2-RR

  WB result of FGFBP1 antibody (85879-2-RR; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-FGFBP1 transfected BxPC-3 cells.

• KD/KO Validated

FGFBP1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85879-2-RR

Clone No.250194B7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

17 kDa HBGF binding protein, 17 kDa HBGF-binding protein, 17 kDa heparin-binding growth factor-binding protein, FGF binding protein 1, FGF BP

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HaCaT cells, mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85879-2-RR targets FGFBP1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6357

  Product name: Recombinant human FGFBP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-234 aa of BC003628

  Sequence: MKICSLTLLSFLLLAAQVLLVEGKKKVKNGLHSKVVSEQKDTLGNTQIKQKSRPGNKGKFVTKDQANCRWAATEQEEGISLKVECTQLDHEFSCVFAGNPTSCLKLKDERVYWKQVARNLRSQKDICRYSKTAVKTRVCRKDFPESSLKLVSSTLFGNTKPRKEKTEMSPREHIKGKETTPSSLAVTQTMATKAPECVEDPDMANQRKTALEFCGETWSSLCTFFLSIVQDTSC

• Full Name: fibroblast growth factor binding protein 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC003628
• Gene Symbol: FGFBP1
• Gene ID (NCBI): 9982
• RRID: AB_3744441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14512
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGFBP1, also named as HBP17, is prominent for its role as the chaperone for fibroblast growth factor-2 (FGF-2), which plays a crucial role in angiogenesis as well as promoting tumor growth. HBp17/FGFBP-1 has been proposed as a candidate biomarker for a number of cancers since it is frequently found to be elevated in many cancer types including in the tissue and cell lines of oral squamous cell carcinomas (OSCC).