FGFBP2 Polyclonal antibody

FGFBP2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 13254-1-AP

Synonyms

HBp17-related protein, Fibroblast growth factor-binding protein 2, FGFBP-2, FGF-BP2, FGF-binding protein 2

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 13254-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13254-1-AP (FGFBP2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 13254-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13254-1-AP (FGFBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of BxPC-3 using 13254-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using FGFBP2 antibody (13254-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, BxPC-3 cells
• Positive IF/ICC detected in: BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

13254-1-AP targets FGFBP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGFBP2 fusion protein Ag4036
• Full Name: fibroblast growth factor binding protein 2
• Calculated Molecular Weight: 223 aa, 28 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC025720
• Gene Symbol: FGFBP2
• Gene ID (NCBI): 83888
• RRID: AB_2103095
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor-binding protein 2 (FGFBP2), also known as 37 kDa killer-specific secretory protein (KSP37), is a member of the fibroblast growth factor binding protein family. FGFBP2 was identified as a Th1/Tc1 specific secretory protein is expressed preferentially in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells and might be involved in essential processes of CTL-mediated immunity. An increase expression of FGFBP2 may be associated with atopic asthma and mild extrinsic asthma. Recently, an observation revealed that FGFBP2 correlate strongly with histology, stage and outcome in ovarian, and it might be a potential prognostic biomarker. The observed MW of FGFBP2 (37 kDa) varies from the predicated MW of 28 kDa, which may be due to the specific space conformation caused by internal disulfide bonds.

Publications

Species	Application	Title
human	IF	Cell Biosci; Single-cell RNA sequencing analysis of the temporomandibular joint condyle in 3 and 4-month-old human embryos; Authors - Qianqi Zhu