FGFBP3 Polyclonal antibody

FGFBP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24725-1-AP

Synonyms

C10orf13, FGF binding protein 3, FGF BP3, FGFBP 3, FGFBP3

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Validation Data Gallery

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  WB analysis using 24725-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24725-1-AP (FGFBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 24725-1-AP

  Immunofluorescent analysis of A431 cells using 24725-1-AP (FGFBP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24725-1-AP is FGFBP3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGFBP3 fusion protein Ag20573
• Full Name: fibroblast growth factor binding protein 3
• Calculated Molecular Weight: 258 aa, 28 kDa
• Observed Molecular Weight: 60-65 kda
• GenBank Accession Number: BC025966
• Gene Symbol: FGFBP3
• Gene ID (NCBI): 143282
• RRID: AB_2879691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGFBP3 also named as C10orf13 or FGF binding protein 3 is 258 amino acid protein, which belongs to the fibroblast growth factor binding family. FGFBP3 is a secreted protein and may play a role in the regulation of gene transcription. The molecular weight of FGFBP3 is 29kDa, but our results detected the 60-65kDa protein by western blot. We infer that FGFBP3 may exist as dimer.