FGFR2/CD332 Recombinant antibody, PBS Only

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  WB analysis using 84205-4-RR (same clone as 84205-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84205-4-RR (FGFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84205-4-RR (same clone as 84205-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84205-4-RR (FGFR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84205-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84205-4-RR against Human FGFR2 were performed. The affinity constant is 18.8 nM.

FGFR2/CD332 Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84205-4-PBS

Clone No.241383G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

FGFR2, 241383G9, BEK, BFR 1, CD332

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Product Information

84205-4-PBS targets FGFR2/CD332 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FGFR2/CD332 fusion protein Eg1265
• Full Name: fibroblast growth factor receptor 2
• Calculated Molecular Weight: 92kd
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: NM_000141.4
• Gene Symbol: FGFR2
• Gene ID (NCBI): 2263
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P21802
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.