Fibrinogen Gamma Chain Monoclonal antibody

Fibrinogen Gamma Chain Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2B2B8

Cat no : 66158-1-Ig

Synonyms

FGG, fibrinogen gamma chain



Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inhuman liver tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

66158-1-Ig targets Fibrinogen Gamma Chain in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Fibrinogen Gamma Chain fusion protein Ag8817
Full Name fibrinogen gamma chain
Calculated molecular weight453aa,52 kDa; 437aa,50 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007044
Gene symbol FGG
Gene ID (NCBI) 2266
Conjugate Unconjugated
Form Liquid
Purification Method Thiophilic Affinity Chromatography
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGG is the gamma chain of fibrinogen. Mutations in the gene of FGG lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia and thrombophilia.

Protocols

Product Specific Protocols
WB protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
IHC protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
IF protocol for Fibrinogen Gamma Chain antibody 66158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Mol Ther Nucleic Acids

Antithrombotic Effect of shRNA Target F12 Mediated by Adeno-Associated Virus.

Authors - Fanfan Li