FH Polyclonal antibody

FH Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11375-1-AP

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1



Tested Applications

Positive WB detected inHEK-293 cells, human placenta tissue, Hela cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11375-1-AP targets FH in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity C. elegans, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FH fusion protein Ag1934
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC003108
Gene symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 11375-1-APDownload protocol
IHC protocol for FH antibody 11375-1-APDownload protocol
IF protocol for FH antibody 11375-1-APDownload protocol
IP protocol for FH antibody 11375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

Anti-senescence role of heterozygous fumarate hydratase gene knockout in rat lung fibroblasts in vitro.

Authors - Zhirui Fan
humanIHC

Thorac Cancer

Bioinformatics analysis and expression study of fumarate hydratase in lung cancer.

Authors - Zongjuan Ming
C. elegansWB

Int J Radiat Biol

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

Authors - Yongyan Sun
humanWB

Invest Ophthalmol Vis Sci

Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.

Authors - Coupland Sarah E SE
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
humanWB

Theranostics

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.

Authors - Feng Shi