Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human placenta tissue, human colon tissue, human kidney tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, caenorhabditis elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1934 Product name: Recombinant human FH protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 204-510 aa of BC003108 Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK Predict reactive species |
| Full Name | fumarate hydratase |
| Calculated Molecular Weight | 510 aa, 55 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC003108 |
| Gene Symbol | FH |
| Gene ID (NCBI) | 2271 |
| RRID | AB_763171 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07954 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FH antibody 11375-1-AP | Download protocol |
| IHC protocol for FH antibody 11375-1-AP | Download protocol |
| IP protocol for FH antibody 11375-1-AP | Download protocol |
| WB protocol for FH antibody 11375-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FH Polyclonal antibody (Cat# 11375-1-AP) has 31 citations. Journals include Nature, Nature Communications, Molecular Cell, Cell Reports, Journal of Clinical Investigation, Cell Death & Disease, Cancer Letters, Theranostics, Metabolism, Aging, Oncotarget, Journal of Biological Chemistry, Journal of Immunology, EMBO Journal, and Advanced Science. Research fields span metabolic reprogramming, TCA cycle/succinate metabolism, cancer (renal, colorectal, glioma, melanoma, bladder, lung), atherosclerosis, mitochondrial homeostasis, NAFLD, heart regeneration, and neurodegeneration/tau pathology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. | ||
Metabolism Glutamine synthetase deficiency triggers mitochondrial oxidative stress and a ribotoxic stress response during acute hepatic metabolic crisis. | ||
Mol Cell Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle. | ||
Theranostics EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. |
Reviews
What customers say
Both reviewers gave this product a perfect 5-star rating. One user noted that a lab colleague used it for Western Blot with no complaints, while the other confirmed the antibody works well for both Western Blot and Immunofluorescence. Overall, customers report reliable performance and satisfaction with the product across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Cynthia (Verified Customer) (01-30-2026) | My lab mate used this and there were no complaints.
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Jia Yi (Verified Customer) (08-22-2025) | This antibodies work well.
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