FH Polyclonal antibody

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  WB analysis of HepG2 using 11375-1-AP

  WB result of FH antibody (11375-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

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  WB analysis using 11375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11375-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11375-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 11375-1-AP

  IP result of anti-FH (IP:11375-1-AP, 4ug; Detection:11375-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.

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  IHC staining of human placenta using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 11375-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11375-1-AP (FH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11375-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FH antibody (11375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11375-1-AP

Publications(21)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HsFH, Fumarase, EC:4.2.1.2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human placenta tissue, human normal colon, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, caenorhabditis elegans
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1934

  Product name: Recombinant human FH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 204-510 aa of BC003108

  Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

• Full Name: fumarate hydratase
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC003108
• Gene Symbol: FH
• Gene ID (NCBI): 2271
• RRID: AB_763171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P07954
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.; Authors - Xiaoyu Ma
mouse	WB	Aging Dis; Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging; Authors - Minghao Yuan
human	WB	Theranostics; EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.; Authors - Feng Shi
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
human	WB	Invest Ophthalmol Vis Sci; Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.; Authors - Coupland Sarah E SE
human	WB	Cell Biochem Biophys; sPLA2-IB and PLA2R Mediate Aberrant Glucose Metabolism in Podocytes via Hyperactivation of the mTOR/HIF-1α Pathway; Authors - Jiwen Bao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jia Yi (Verified Customer) (08-22-2025)

This antibodies work well.

• Applications: Western Blot, Immunofluorescence