FH Polyclonal antibody

FH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11375-1-AP

Synonyms

FH, Fumarase, fumarate hydratase, HLRCC, LRCC, MCL, MCUL1



Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue, human placenta tissue, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman ovary tumor tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11375-1-AP targets FH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity C. elegans, human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FH fusion protein Ag1934
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC003108
Gene symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 11375-1-APDownload protocol
IHC protocol for FH antibody 11375-1-APDownload protocol
IF protocol for FH antibody 11375-1-APDownload protocol
IP protocol for FH antibody 11375-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Aging (Albany NY)

Anti-senescence role of heterozygous fumarate hydratase gene knockout in rat lung fibroblasts in vitro.

Authors - Zhirui Fan
humanIHC

Thorac Cancer

Bioinformatics analysis and expression study of fumarate hydratase in lung cancer.

Authors - Zongjuan Ming
C. elegansWB

Int J Radiat Biol

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.

Authors - Yongyan Sun
humanWB

Invest Ophthalmol Vis Sci

Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.

Authors - Coupland Sarah E SE
humanWB

Oncotarget

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Authors - Zhichao Gu
humanWB

Theranostics

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.

Authors - Feng Shi