FH Monoclonal antibody, PBS Only

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  WB analysis using 68162-1-Ig (same clone as 68162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 68162-1-Ig (same clone as 68162-1-PBS)

  WB result of FH antibody (68162-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells. This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 68162-1-Ig (same clone as 68162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68162-1-Ig (FH antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68162-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

FH Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 68162-1-PBS

Clone No.1A9C8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

HsFH, Fumarase, EC:4.2.1.2, 1A9C8

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Product Information

68162-1-PBS targets FH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30254

  Product name: Recombinant human FH protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 204-510 aa of BC003108

  Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

• Full Name: fumarate hydratase
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC003108
• Gene Symbol: FH
• Gene ID (NCBI): 2271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07954
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.