FH Recombinant monoclonal antibody

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  WB analysis of HepG2 using 82508-1-RR

  WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

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  WB analysis using 82508-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 82508-1-RR

  IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug.

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  IHC staining of human stomach using 82508-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82508-1-RR (FH antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 82508-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82508-1-RR (FH antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82508-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

FH Uni-rAb^® Recombinant Antibody for WB, IHC, IP, ELISA

Cat No. 82508-1-RR

Clone No.3K16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

3K16, EC:4.2.1.2, Fumarase, HsFH

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, A431 cells, L02 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human stomach tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82508-1-RR targets FH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1934

  Product name: Recombinant human FH protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 204-510 aa of BC003108

  Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

• Full Name: fumarate hydratase
• Calculated Molecular Weight: 510 aa, 55 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC003108
• Gene Symbol: FH
• Gene ID (NCBI): 2271
• RRID: AB_3086485
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07954
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.