FHIT Monoclonal antibody

FHIT Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4D6G1

Cat no : 66086-1-Ig

Synonyms

AP3A hydrolase, AP3Aase, Bis(5 adenosyl) triphosphatase, Dinucleosidetriphosphatase, FHIT, FRA3B, fragile histidine triad gene



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66086-1-Ig is FHIT Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FHIT fusion protein Ag14471
Full Name fragile histidine triad gene
Calculated molecular weight 147 aa, 17 kDa
Observed molecular weight 17 kDa
GenBank accession numberBC032336
Gene symbol FHIT
Gene ID (NCBI) 2272
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.

Protocols

Product Specific Protocols
WB protocol for FHIT antibody 66086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Int J Clin Exp Pathol

Upregulation of FHIT gene expression in endometrial carcinoma by RNA activation.

Authors - Qing Zhu
humanIHC

Cancer Biomark

FHIT and C-MYC expression in cervical histology and cytology as biomarkers for detecting high-grade intraepithelial neoplasia in human papillomavirus-positive women.

Authors - Fan Yang