Multi-rAb® FHL2 Multi-Recombinant antibody

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• Multi-rAb® FHL2 Multi-Recombinant antibody (RMX00084)

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  WB analysis using RMX00084

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00084 (FHL2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using RMX00084

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00084 (FHL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using RMX00084

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using RMX00084 (FHL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HSkMC using RMX00084

  Immunofluorescent analysis of (-20°C Ethanol) fixed HSkMC cells using FHL2 antibody (RMX00084) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

FHL2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00084

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

AAG11, DRAL, FHL 2, FHL-2, LIM domain protein DRAL

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Tested Applications

• Positive WB detected in: HeLa cells, U2OS cells, NIH/3T3 cells, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HSkMC cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00084 targets FHL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: four and a half LIM domains 2
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC014397
• Gene Symbol: FHL2
• Gene ID (NCBI): 2274
• RRID: AB_3750133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q14192
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FHL2(Four and a half LIM domains protein 2), also known as DRAL. It is located in cytoplasm and nucleus. The protein is expressed in skeletal muscle and heart. May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation. Negatively regulates the calcineurin/NFAT signaling pathway in cardiomyocytes (PMID: 28717008). The molecular weight of FHL2 is 32 kDa.