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FHOD3 Polyclonal antibody, PBS Only (31744-1-PBS)

Filter: AllIHCIF-P

IHC staining of mouse heart using 31744-1-AP (same clone as 31744-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31744-1-AP (FHOD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31744-1-PBS in a different storage buffer formulation.
IHC staining of mouse heart using 31744-1-AP (same clone as 31744-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 31744-1-AP (FHOD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31744-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 31744-1-AP (same clone as 31744-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using FHOD3 antibody (31744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31744-1-PBS in a different storage buffer formulation.
IF Staining of mouse heart using 31744-1-AP (same clone as 31744-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using FHOD3 antibody (31744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31744-1-PBS in a different storage buffer formulation.

FHOD3 Polyclonal antibody, PBS Only

Name: FHOD3 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 31744-1-PBS

FHOD3 Polyclonal Antibody for IHC, IF-P, Indirect ELISA

Cat No. 31744-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, Indirect ELISA

Synonyms

CMH28, FHOS2, hFHOS2, KIAA1695

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

31744-1-PBS targets FHOD3 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag36247 Product name: Recombinant human FHOD3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 650-825 aa of BC157093 Sequence: RTTGLWPAGVQDAGVNGQCGDILTNKRFMLDMLYAHNRKSPDDEEKGDGEAGRTQQEAEAVASLATRISTLQANSQTQDESVRRVDVGCLDNRGSVKAFAEKFNSGDLGRGSISPDAEPNDKVPETAPVQPKTESDYIWDQLMANPRELRIQDMDFTDLGEEDDIDVLDVDLGHRE Predict reactive species
Full Name	formin homology 2 domain containing 3
GenBank Accession Number	BC157093
Gene Symbol	FHOD3
Gene ID (NCBI)	80206
RRID	AB_3742470
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q2V2M9
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

FHOD3, also known as FHOS2 or Formactin2, was an important member of Formin homology proteins. FHOD3 plays a crucial role in organizing the cytoskeleton, primarily by regulating actin filament polymerization within cardiomyocytes, which is essential for heart muscle structure and function. Mutations in this gene are associated with familial cardiomyopathies, including dilated cardiomyopathy (DCM), and its protein levels are altered in hypertrophic cardiomyopathy (HCM). FHOD3 is expressed abundantly in the heart (PMID: 32447646, PMID: 33251537).