FIBP Polyclonal antibody

FIBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15968-1-AP

Synonyms

FGFIBP, FIBP, FIBP 1

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Validation Data Gallery

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  WB analysis of BxPC-3 using 15968-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15968-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse pancreas using 15968-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  WB analysis of BxPC-3 using 15968-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15968-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15968-1-AP

  Immunofluorescent analysis of HepG2 cells using 15968-1-AP (FIBP antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, mouse pancreas tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15968-1-AP targets FIBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FIBP fusion protein Ag8743
• Full Name: fibroblast growth factor (acidic) intracellular binding protein
• Calculated Molecular Weight: 357 aa, 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC014388
• Gene Symbol: FIBP
• Gene ID (NCBI): 9158
• RRID: AB_2878203
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FIBP, also named as Acidic fibroblast growth factor intracellular binding protein, is a 364 amino acid protein, which is highly expressed in heart, skeletal muscle and pancreas. FIBP may be involved in mitogenic function of FGF1.