FICD Polyclonal antibody

FICD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11974-1-AP

Synonyms

AMPylator FICD, FIC domain containing, FIC domain containing protein, FICD, HIP 13, HIP13, Huntingtin yeast partner E, HYPE, UNQ3041

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Validation Data Gallery

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  WB analysis of A549 using 11974-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11974-1-AP (FICD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11974-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11974-1-AP (FICD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human small intestine using 11974-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 11974-1-AP (FICD Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells
• Positive IHC detected in: human small intestine tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11974-1-AP targets FICD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FICD fusion protein Ag2597
• Full Name: FIC domain containing
• Calculated Molecular Weight: 458 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC001342
• Gene Symbol: FICD
• Gene ID (NCBI): 11153
• RRID: AB_2877811
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenosine monophosphate-protein transferase FICD, also named as HIP13 or HYPE, is a 458 amino acid protein, which contains one fido domain and two TPR repeats. FICD localizes in the membrane and belongs to the fic family. FICD as an adenylyltransferase that mediates the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins. The fido domain mediates the adenylyltransferase activity