FIGNL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FIGNL1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17604-1-AP

Synonyms

Fidgetin-like protein 1, EC:3.6.4.-

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Validation Data Gallery

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  WB analysis using 17604-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17604-1-AP (FIGNL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17604-1-AP

  IP result of anti-FIGNL1 (IP:17604-1-AP, 4ug; Detection:17604-1-AP 1:1000) with HeLa cells lysate 2680ug.

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  IF Staining of HeLa using 17604-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17604-1-AP (FIGNL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HuH-7 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17604-1-AP targets FIGNL1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FIGNL1 fusion protein Ag11709
• Full Name: fidgetin-like 1
• Calculated Molecular Weight: 674 aa, 74 kDa
• Observed Molecular Weight: 66-71 kDa
• GenBank Accession Number: BC051867
• Gene Symbol: FIGNL1
• Gene ID (NCBI): 63979
• RRID: AB_2104675
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,CoIP	Cell Rep; C1orf112 teams up with FIGNL1 to facilitate RAD51 filament disassembly and DNA interstrand cross-link repair; Authors - Zenan Zhou ; KD Validated
human	WB	Int J Oncol; FIGNL1 promotes non‑small cell lung cancer cell proliferation.; Authors - Miao Li ; KD Validated
human	WB,IHC	Curr Gene Ther; FIGNL1 Promotes Hepatocellular Carcinoma Formation via Remodeling ECM-receptor Interaction Pathway Mediated by HMMR; Authors - Jiabei Wang
human	WB	Oncol Rep; PMID: 28260065; Authors - Jian Ma ; KD Validated
human	WB,IP	bioRxiv; RADIF(C1orf112)-FIGNL1 Complex Regulates RAD51 Chromatin Association to Promote Viability After Replication Stress; Authors - Jessica D Tischler
human	WB	Nat Commun; FLIP(C1orf112)-FIGNL1 complex regulates RAD51 chromatin association to promote viability after replication stress; Authors - Jessica D Tischler