FIP1L1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83863-2-RR (same clone as 83863-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83863-2-RR (FIP1L1 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 83863-2-RR (same clone as 83863-2-PBS)

  WB result of FIP1L1 antibody (83863-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FIP1L1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 83863-2-RR (same clone as 83863-2-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

• 

  FC experiment of U-2 OS using 83863-2-RR (same clone as 83863-2-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-2-RR, Clone:240658C4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83863-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83863-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83863-2-RR against Human FIP1L1 were performed. The affinity constant is 10.8 pM.

• Featured Product
• KD/KO Validated

FIP1L1 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83863-2-PBS

Clone No.240658C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

240658C4, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

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Product Information

83863-2-PBS targets FIP1L1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2921

  Product name: Recombinant human FIP1L1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC017724

  Sequence: MSAGEVERLVSELSGGTGGDEEEEWLYGDENEVERPEEENASANPPSGIEDETAENGVPKPKVTETEDDSDSDSDDDEDDVHVTIGDIKTGAPQYGSYGTAPVNLNIKTGGRVYGTTGTKVKGVDLDAPGSINGVPLLEVDLDSFEDKPWRKPGADLSDYFNYGFNEDTWKAYCEKQKRIRMGLEVIPVTSTTNKITAEDCTMEVTPGAEIQDGRFNLFKVQQGRTGNSEKETALPSTKAEFTSPPSLFKTGLPPSRNSTSSQSQTSTASRKANSSVGKWQDRYGRAESPDLRRLPGAIDVIGQTITISRVEGRRRANENSNIQVLSERSATEVDNNFSKPPPFFPPGAP

• Full Name: FIP1 like 1 (S. cerevisiae)
• Calculated Molecular Weight: 594 aa, 66 kDa
• Observed Molecular Weight: 67-80 kDa
• GenBank Accession Number: BC017724
• Gene Symbol: FIP1L1
• Gene ID (NCBI): 81608
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q6UN15
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).