FJX1 Polyclonal antibody

FJX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17417-1-AP

Synonyms

FJX1, Four jointed box protein 1, Four jointed protein homolog

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Validation Data Gallery

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  WB analysis of rat brain using 17417-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17417-1-AP (FJX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17417-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17417-1-AP (FJX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IHC detected in: mouse brain tissue, human liver tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17417-1-AP targets FJX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FJX1 fusion protein Ag11385
• Full Name: four jointed box 1 (Drosophila)
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC049171
• Gene Symbol: FJX1
• Gene ID (NCBI): 24147
• RRID: AB_2878406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Four-jointed box protein 1 (FJX1), also named as FLJ22416, is a 437 amino acid protein, which belongs to the FJX1 family. FJX1 is a secreted protein and acts as an inhibitor of dendrite and branching. FJX1 is important for growth and differentiation of legs and wings, and for proper development of the eyes. FAT4, an essential gene that has a key role in vertebrate PCP (planar cell polarity), represses FJX1 expression which may lead to cystic diseases in humans.

Publications

Species	Application	Title
rat	WB	Mol Cell Endocrinol; Regulation of Sertoli cell function by planar cell polarity (PCP) protein Fjx1; Authors - Tiao Bu
rat	WB	Reprod Biol Endocrinol; Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin; Authors - Lingling Wang
human	WB	Endocrinology; The planar cell polarity (PCP) protein Fat 1 in Sertoli cell function; Authors - Tiao Bu