FKBP10/FKBP65 Monoclonal antibody

FKBP10/FKBP65 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D9

Cat no : 68085-1-Ig

Synonyms

65 kDa FK506 binding protein, 65 kDa FKBP, FK506 binding protein 10, FKBP 10, FKBP 65, FKBP10, FKBP6, FKBP65, FLJ20683, FLJ22041, FLJ23833, hFKBP65, Immunophilin FKBP65, PPIase FKBP10, Rotamase

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Validation Data Gallery

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  WB analysis using 68085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68085-1-Ig (FKBP10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68085-1-Ig targets FKBP10/FKBP65 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBP10/FKBP65 fusion protein Ag31628
• Full Name: FK506 binding protein 10, 65 kDa
• Calculated Molecular Weight: 582 aa, 64 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC016467
• Gene Symbol: FKBP10
• Gene ID (NCBI): 60681
• RRID: AB_2918822
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.