FKBP14 Polyclonal antibody

FKBP14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 15884-1-AP

Synonyms

FKBP-22, FKBP22, FKBP-14, FK506-binding protein 14, EC:5.2.1.8

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Validation Data Gallery

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  WB analysis of HEK-293 using 15884-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15884-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15884-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15884-1-AP (FKBP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 15884-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15884-1-AP (FKBP14 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 3 publications below

Product Information

15884-1-AP targets FKBP14 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP14 fusion protein Ag8668
• Full Name: FK506 binding protein 14, 22 kDa
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC005206
• Gene Symbol: FKBP14
• Gene ID (NCBI): 55033
• RRID: AB_2102702
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP14, also named as FKBP22, belongs to the family of FK506-binding peptidyl-prolyl cis-trans isomerases (PPIases). PPIases accelerate the folding of proteins during protein synthesis. FKBP14 is localized in the endoplasmic reticulum (ER) and that deficiency of FKBP14 leads to enlarged ER cisterns in dermal fibroblasts in vivo. FKBP14 is about 22kd.

Publications

Species	Application	Title
human	WB,IF	Am J Hum Genet; Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.; Authors - Baumann Matthias M
human	WB,IF	Sci Rep; The novel missense mutation Met48Lys in FKBP22 changes its structure and functions.; Authors - Yoshihiro Ishikawa
human	IF	Hum Mutat; Kyphoscoliotic Ehlers-Danlos syndrome caused by pathogenic variants in FKBP14: Further insights into the phenotypic spectrum and pathogenic mechanisms; Authors - Birute Burnyte
human	WB	Int J Mol Sci; Local Net Charge State of Collagen Triple Helix Is a Determinant of FKBP22 Binding to Collagen III; Authors - Yoshihiro Ishikawa