FKBP52 Polyclonal antibody

FKBP52 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22623-1-AP

Synonyms

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

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Validation Data Gallery

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  WB analysis of PC-3 using 22623-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22623-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-3 using 22623-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22623-1-AP (FKBP52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22623-1-AP targets FKBP52 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP52 fusion protein Ag18704
• Full Name: FK506 binding protein 4, 59kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007924
• Gene Symbol: FKBP4
• Gene ID (NCBI): 2288
• RRID: AB_2879136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The large molecular-weight immunophilin, FKBP52, is a known target of the immunosuppressive drug FK506. FKBP52 exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is inhibited by the binding of FK506-properties that it shares with the smaller but better-studied immunophilin, FKBP12(PMID:15381148). It is essential to AR-mediated induction of genes involved in sexually dimorphic development of the penis (NKX3-1), in erectile function of the mature organ (NOS2 and NOS3), and in promotion of apoptosis during development(PMID:20605780).