Fukutin Polyclonal antibody

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  WB analysis of human brain using 18276-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18276-1-AP (Fukutin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18276-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18276-1-AP (Fukutin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18276-1-AP

  IP result of anti-Fukutin (IP:18276-1-AP, 4ug; Detection:18276-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas using 18276-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18276-1-AP (Fukutin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 18276-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18276-1-AP (Fukutin antibody) at dilution of 1:100 (under 40x lens).

Fukutin Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 18276-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

CMD1X, FCMD, FKTN, Fukutin, LGMD2M

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Tested Applications

• Positive WB detected in: human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

18276-1-AP targets Fukutin in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13067

  Product name: Recombinant human FKTN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 35-461 aa of BC101808

  Sequence: KNGAGLSKSKGSRIGFDSTQWRAVKKFIMLTSNQNVPVFLIDPLILELINKNFEQVKNTSHGSTSQCKFFCVPRDFTAFALQYHLWKNEEGWFRIAENMGFQCLKIESKDPRLDGIDSLSGTEIPLHYICKLATHAIHLVVFHERSGNYLWHGHLRLKEHIDRKFVPFRKLQFGRYPGAFDRPELQQVTVDGLEVLIPKDPMHFVEEVPHSRFIECRYKEARAFFQQYLDDNTVEAVAFRKSAKELLQLAAKTLNKLGVPFWLSSGTCLGWYRQCNIIPYSKDVDLGIFIQDYKSDIILAFQDAGLPLKHKFGKVEDSLELSFQGKDDVKLDVFFFYEETDHMWNGGTQAKTGKKFKYLFPKFTLCWTEFVDMKVHVPCETLEYIEANYGKTWKIPVKTWDWKRSPPNVQPNGIWPISEWDEVIQLY

• Full Name: fukutin
• Calculated Molecular Weight: 461 aa, 54 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC101808
• Gene Symbol: Fukutin
• Gene ID (NCBI): 2218
• RRID: AB_2103278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75072
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell Proteomics; Direct mapping of additional modifications on phosphorylated O-glycans of α-dystroglycan by mass spectrometry analysis in conjunction with knocking out of causative genes for dystroglycanopathy.; Authors - Hirokazu Yagi