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FLAD1 Polyclonal antibody

FLAD1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14118-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

FADS, FAD1, FAD synthase region, FAD synthase, FAD pyrophosphorylase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HepG2 cells, rat liver tissue, L02 cells, mouse liver tissue
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14118-1-AP targets FLAD1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5267

Product name: Recombinant human FLAD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 148-447 aa of BC011378

Sequence: LVSVRNVYLFPGIPELLRRVLEGMKGLFQNPAVQFHSKELYVAADEASIAPILAEAQAHFGRRLGLGSYPDWGSNYYQVKLTLDSEEEGPLEECLAYLTARLPQGSLVPYMPNAVEQASEAVYKLAESGSSLGKKVAGALQTIETSLAQYSLTQLCVGFNGGKDCTALLHLFHAAVQRKLPDVPNPLQILYIRSISPFPELEQFLQDTIKRYNLQMLEAEGSMKQALGELQARHPQLEAVLMGTRRTDPYSCSLCPFSPTDPGWPAFMRINPLLDWTYRDIWDFLRQLFVPYCILYDRG

Predict reactive species
Full Name FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae)
Calculated Molecular Weight 446 aa, 49 kDa
Observed Molecular Weight 50 kDa, 65-70 kDa
GenBank Accession NumberBC011378
Gene Symbol FLAD1
Gene ID (NCBI) 80308
RRIDAB_2878017
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFF5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLAD1 is a protein-coding gene for flavin adenine dinucleotide synthetase (FADS), a key enzyme in the FAD biosynthesis process which contains an N-terminal molybdopterin -binding (MPTb) domain and a C-terminal domain (FADS domain) (PMID: 32714079). Alternative splicing of the human FLAD1 gene generates different isoforms of the enzyme FAD synthase. ~60 and ~50 kDa bands correspond to the expected mitochondrial FADS1 and cytosolic FADS2 proteins, respectively (PMID: 29316637).

Protocols

Product Specific Protocols
WB protocol for FLAD1 antibody 14118-1-APDownload protocol
IF protocol for FLAD1 antibody 14118-1-APDownload protocol
IP protocol for FLAD1 antibody 14118-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Anal Chem

Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.

Authors - Hongwei Chu
mouseWB

Ecotoxicol Environ Saf

Di (2-ethylhexyl) phthalate reduces sperm motility by decreasing sperm tail energy supply

Authors - Xiao-Lu Li
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