FLNA Monoclonal antibody

FLNA Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

CloneNo.

1G4H3

Cat no : 67133-1-Ig

Synonyms

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

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Validation Data Gallery

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  WB analysis using 67133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67133-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 67133-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67133-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 67133-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67133-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of HeLa using 67133-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, MCF-7 cells, human skeletal muscle tissue, U2OS cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, A431 cells
• Positive IHC detected in: human cervical cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

67133-1-Ig targets FLNA in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FLNA fusion protein Ag23291
• Full Name: filamin A, alpha (actin binding protein 280)
• Calculated Molecular Weight: 2647 aa, 280 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: BC014654
• Gene Symbol: FLNA
• Gene ID (NCBI): 2316
• RRID: AB_2882432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)

Publications

Species	Application	Title
human	WB,IF	Front Cell Dev Biol; NudC L279P Mutation Destabilizes Filamin A by Inhibiting the Hsp90 Chaperoning Pathway and Suppresses Cell Migration.; Authors - Min Liu
human	IF	Hum Mol Genet; Expression and subcellular localization of USH1C/harmonin in human retina provides insights into pathomechanisms and therapy; Authors - Kerstin Nagel-Wolfrum
human	IHC	Mol Med Rep; Filamin A regulates EGFR/ERK/Akt signaling and affects colorectal cancer cell growth and migration.; Authors - Kun Wang
human	WB	Cell Death Dis; NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90.; Authors - Wenwen Chen
human	IF	J Exp Clin Cancer Res; Focal Adhesion Kinase (FAK)-Hippo/YAP transduction signaling mediates the stimulatory effects exerted by S100A8/A9-RAGE system in triple-negative breast cancer (TNBC).; Authors - Damiano Cosimo Rigiracciolo
human	WB	Cell Death Dis; FRMD3 inhibits the growth and metastasis of breast cancer through the ubiquitination-mediated degradation of vimentin and subsequent impairment of focal adhesion; Authors - Wenjun Shao