Flotillin 1 Polyclonal antibody

Flotillin 1 Polyclonal Antibody for FC, IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 15571-1-AP

Synonyms

FLOT1, flotillin 1



Tested Applications

Positive WB detected inmouse brain tissue, rat heart tissue, mouse heart tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IF detected inHela cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15571-1-AP targets Flotillin 1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Flotillin 1 fusion protein Ag7902
Full Name flotillin 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC001146
Gene symbol FLOT1
Gene ID (NCBI) 10211
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.

Protocols

Product Specific Protocols
WB protocol for Flotillin 1 antibody 15571-1-APDownload protocol
IF protocol for Flotillin 1 antibody 15571-1-APDownload protocol
IP protocol for Flotillin 1 antibody 15571-1-APDownload protocol
FC protocol for Flotillin 1 antibody 15571-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIP,WB

J Ethnopharmacol

Shenmai injection maintains blood-brain barrier integrity following focal cerebral ischemia via modulating the expression and trafficking of occludin in lipid rafts.

Authors - Huaming Xu
humanWB

Transplant Direct

Circulating Donor Heart Exosome Profiling Enables Noninvasive Detection of Antibody-mediated Rejection.

Authors - Robert W Hu
humanWB

Biomed Res Int

Circulating Exosomal miRNAs as Novel Biomarkers for Stable Coronary Artery Disease.

Authors - Ping Zhang
humanWB

Int J Mol Sci

GBA Mutations Influence the Release and Pathological Effects of Small Extracellular Vesicles from Fibroblasts of Patients with Parkinson's Disease.

Authors - Silvia Cerri
humanWB

Cardiol Young

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Authors - Li Zhang
IF

J Reprod Dev

Discovery of new receptors regulating LH and FSH secretion by bovine gonadotrophs to explore a new paradigm for mechanisms regulating reproduction.

Authors - Hiroya Kadokawa