|Positive WB detected in||mouse brain tissue, rat heart tissue, mouse heart tissue, rat brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IF detected in||Hela cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
15571-1-AP targets Flotillin 1 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Flotillin 1 fusion protein Ag7902|
|Full Name||flotillin 1|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC001146|
|Gene ID (NCBI)||10211|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
|Product Specific Protocols|
|WB protocol for Flotillin 1 antibody 15571-1-AP||Download protocol|
|IF protocol for Flotillin 1 antibody 15571-1-AP||Download protocol|
|IP protocol for Flotillin 1 antibody 15571-1-AP||Download protocol|
|FC protocol for Flotillin 1 antibody 15571-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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