Tested Applications
| Positive WB detected in | mouse brain tissue, U-251 cells, mouse heart tissue, rat brain tissue, rat heart tissue, U-87 MG cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15571-1-AP targets Flotillin 1 in WB, IHC, IF/ICC, IP, CoIP, ELISA, IB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7902 Product name: Recombinant human Flotillin 1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 80-427 aa of BC001146 Sequence: MLAAACQMFLGKTEAEIAHIALETLEGHQRAIMAHMTVEEIYKDRQKFSEQVFKVASSDLVNMGISVVSYTLKDIHDDQDYLHSLGKARTAQVQKDARIGEAEAKRDAGIREAKAKQEKVSAQYLSEIEMAKAQRDYELKKAAYDIEVNTRRAQADLAYQLQVAKTKQQIEEQRVQVQVVERAQQVAVQEQEIARREKELEARVRKPAEAERYKLERLAEAEKSQLIMQAEAEAASVRMRGEAEAFAIGARARAEAEQMAKKAEAFQLYQEAAQLDMLLEKLPQVAEEISGPLTSANKITLVSSGSGTMGAAKVTGEVLDILTRLPESVERLTGVSISQVNHKPLRTA Predict reactive species |
| Full Name | flotillin 1 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 47 kDa |
| GenBank Accession Number | BC001146 |
| Gene Symbol | Flotillin 1 |
| Gene ID (NCBI) | 10211 |
| RRID | AB_2106746 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins commonly used as lipid raft markers. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IHC protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| IP protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| WB protocol for Flotillin 1 antibody 15571-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Flotillin 1 (FLOT1) polyclonal antibody (Cat# 15571-1-AP) has 76 citations published in journals including Nature Communications, Cell Research, Gastroenterology, PNAS, Theranostics, Advanced Science, ACS Nano, and Science Reports. Associated research fields encompass extracellular vesicle/exosome biology, oncology (glioblastoma, pancreatic, gastric, hepatocellular carcinoma), neurodegeneration (Alzheimer's, Parkinson's), lipid raft signaling, ferroptosis, necroptosis, inflammation, metabolism, immunology, and cardiovascular disease. Western blot is the predominant application.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther GRP75 triggers white adipose tissue browning to promote cancer-associated cachexia | ||
Cell Res Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death. | ||
Gastroenterology Stromal Stiffness-Regulated IGF2BP2 in Pancreatic Cancer Drives Immune Evasion via Sphingomyelin Metabolism | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Nat Commun An attention-demanding hunting paradigm engages the superior colliculus-zona incerta circuit mediating analgesia in male mice. | ||
Nat Commun Cross-linking of the endolysosomal system reveals potential flotillin structures and cargo |
Reviews
What customers say
Both reviewers rated this antibody 4 out of 5 stars for Western Blot. One user tested it on mouse samples at a 1:1000 dilution and reported it "worked well." The other used it on ARPE-19 cells at 1:2500 and noted good performance with a specific band at the expected size, along with two larger, weaker bands. Overall, users find it reliable for WB applications across different sample types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bruna (Verified Customer) (06-10-2026) | worked well
 |
Guorong (Verified Customer) (03-22-2022) | Good performance with a specific band of expected size and 2 bigger and weaker bands
 |
