Tested Applications
| Positive WB detected in | HL-60 cells, THP-1 cells |
| Positive IP detected in | THP-1 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30297-1-AP targets FLT3 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32898 Product name: Recombinant human FLT3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 683-810 aa of BC126350 Sequence: LSGPIYLIFEYCCYGDLLNYLRSKREKFHRTWTEIFKEHNFSFYPTFQSHPNSSMPGSREVQIHPDSDQISGLHGNSFHSEDEIEYENQKRLEEEEDLNVLTFEDLLCFAYQVAKGMEFLEFKSCVHR Predict reactive species |
| Full Name | fms-related tyrosine kinase 3 |
| Calculated Molecular Weight | 112 kDa |
| Observed Molecular Weight | 130 kDa, 150 kDa |
| GenBank Accession Number | BC126350 |
| Gene Symbol | FLT3 |
| Gene ID (NCBI) | 2322 |
| RRID | AB_3086289 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36888 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FLT3 antibody 30297-1-AP | Download protocol |
| IP protocol for FLT3 antibody 30297-1-AP | Download protocol |
| WB protocol for FLT3 antibody 30297-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FLT3 Polyclonal antibody (Cat# 30297-1-AP) has 3 total citations. These appear in the Journal of Medicinal Chemistry (2 papers) and the European Journal of Medicinal Chemistry (1 paper). The associated research fields include FLT3 inhibitor discovery, dual PROTAC degrader development, and novel antitumor compound design targeting tubulin and FLT3 in acute myeloid leukemia. All three studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
J Med Chem Discovery of FYJ-195 as a Highly Potent FLT3 Inhibitor against Multiple Acquired Resistance Mutations in Acute Myeloid Leukemia. | ||
J Med Chem Discovery of Potent Dual PROTAC Degraders Targeting BET-Kinase To Overcome FLT3 Inhibitor Resistance | ||
Eur J Med Chem Discovery of a novel 1H-pyrazole-3-amine derivative 6h with potent antitumor activity by targeting tubulin and FLT3. |
