FLT3 Polyclonal antibody

FLT3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 30297-1-AP

Synonyms

CD135, FL cytokine receptor, FLK2, FLT 3, FLT3, Fms like tyrosine kinase 3, fms related tyrosine kinase 3, Stem cell tyrosine kinase 1, STK 1, STK1

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Validation Data Gallery

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  WB analysis using 30297-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 30297-1-AP (FLT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of THP-1 using 30297-1-AP

  IP result of anti-FLT3 (IP:30297-1-AP, 4ug; Detection:30297-1-AP 1:500) with THP-1 cells lysate 2720 ug.

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IP detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30297-1-AP targets FLT3 in WB, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FLT3 fusion protein Ag32898
• Full Name: fms-related tyrosine kinase 3
• Calculated Molecular Weight: 112 kDa
• Observed Molecular Weight: 130 kDa, 150 kDa
• GenBank Accession Number: BC126350
• Gene Symbol: FLT3
• Gene ID (NCBI): 2322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLT3 (also known as CD135 or FLK2) is a tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation, and survival of hematopoietic progenitor cells and of dendritic cells. FLT3 was originally identified by its expression in hematopoietic stem/progenitor cells (PMID: 7507245). It is important for the normal development of hematopoietic stem/progenitor cells. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.