FMN1 Polyclonal antibody

FMN1 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25982-1-AP

Synonyms

FMN, FMN1, FMN1 Ens, formin 1, LD

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Validation Data Gallery

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  WB analysis of A549 using 25982-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25982-1-AP (FMN1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 25982-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 25982-1-AP (FMN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 25982-1-AP is FMN1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMN1 fusion protein Ag23059
• Full Name: formin 1
• Calculated Molecular Weight: 1196 aa, 132 kDa
• Observed Molecular Weight: 160 kDa
• GenBank Accession Number: BC103692
• Gene Symbol: FMN1
• Gene ID (NCBI): 342184
• RRID: AB_2880319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Molecules; MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway; Authors - Pan Xu