FMNL1 Polyclonal antibody

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• FMNL1 Polyclonal antibody (10395-1-AP)

FMNL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10395-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CLL-associated antigen KW-13, FMNL, Formin-like protein 1

• 

  WB analysis of Raji using 10395-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 10395-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 10395-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10395-1-AP (FMNL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human small intestine using 10395-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10395-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 10395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of Saos-2 using 10395-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed Saos-2 cells using FMNL1 antibody (10395-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells, HeLa cells
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells, Saos-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:500
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10395-1-AP targets FMNL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0594

  Product name: Recombinant human FMNL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 39-351 aa of BC001710

  Sequence: ELDMSDFEEQFKTKSQGPSLDLSALKSKAAQKAPSKATLIEANRAKNLAITLRKGNLGAERICQAIEAYDLQALGLDFLELLMRFLPTEYERSLITRFEREQRPMEELSEEDRFMLCFSRIPRLPERMTTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEIVLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSDLHFLDKAGSVSLDSVLADVRSLQRGLELTQREFVRQDDCMVLKEFLRANSPTMDKLLADSKTAQEAFESVVEYFGENPKTTSP

• Full Name: formin-like 1
• Calculated Molecular Weight: 160 kDa
• Observed Molecular Weight: 150-160 kDa
• GenBank Accession Number: BC001710
• Gene Symbol: FMNL1
• Gene ID (NCBI): 752
• RRID: AB_10895969
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95466
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. The MW of FMNL1 is about 150-160 kDa. (PMID: 16494944 )

Publications

What published studies show

The FMNL1 Polyclonal antibody (Cat# 10395-1-AP) has 1 citation. It appears in Signal Transduction and Targeted Therapy (Impact Factor: 81.2), published in 2026. The cited study, "Region-resolved proteomic map of the human brain: functional interconnections and neurological implications," utilized this antibody for IHC on human samples. The research field is neuroscience, specifically brain proteomics and neurological mapping.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Signal Transduct Target Ther; Region-resolved proteomic map of the human brain: functional interconnections and neurological implications; Authors - Pei-Pei Zhang